Novel in vitro booster vaccination to rapidly generate antigen-specific human monoclonal antibodiesMore about Open Access at the Crick
Authors listIrene Sanjuan Nandin Carol Fong Cecilia Deantonio Juan A Torreno-Pina Simone Pecetta Paula Maldonado Francesca Gasparrini Jose Ordovas-Montanes Samuel W Kazer Svend Kjaer Daryl W Borley Usha Nair Julia A Coleman Daniel Lingwood Alex K Shalek Eric Meffre Pascal Poignard Dennis R Burton Facundo D Batista
Vaccines remain the most effective tool to prevent infectious diseases. Here, we introduce an in vitro booster vaccination approach that relies on antigen-dependent activation of human memory B cells in culture. This stimulation induces antigen-specific B cell proliferation, differentiation of B cells into plasma cells, and robust antibody secretion after a few days of culture. We validated this strategy using cells from healthy donors to retrieve human antibodies against tetanus toxoid and influenza hemagglutinin (HA) from H1N1 and newly emergent subtypes such as H5N1 and H7N9. Anti-HA antibodies were cross-reactive against multiple subtypes, and some showed neutralizing activity. Although these antibodies may have arisen as a result of previous influenza infection, we also obtained gp120-reactive antibodies from non-HIV-infected donors, indicating that we can generate antibodies without prior antigenic exposure. Overall, our novel approach can be used to rapidly produce therapeutic antibodies and has the potential to assess the immunogenicity of candidate antigens, which could be exploited in future vaccine development.
Journal Journal of Experimental Medicine
Issue number 8