Optimisation of a micro-neutralisation assay and its application in antigenic characterisation of influenza virusesMore about Open Access at the Crick
Authors listYipu Lin Yan Gu Stephen A Wharton Lynne Whittaker Victoria Gregory Xiaoyan Li Simon Metin Nicholas Cattle Rodney S Daniels Alan J Hay John Mccauley
The identification of antigenic variants and the selection of influenza viruses for vaccine production are based largely on antigenic characterisation of the haemagglutinin (HA) of circulating viruses using the haemagglutination inhibition (HI) assay. However, in addition to evolution related to escape from host immunity, variants emerging as a result of propagation in different cell substrates can complicate the interpretation of HI results. The objective was to develop further a micro-neutralisation (MN) assay to complement the HI assay in antigenic characterisation of influenza viruses to assess the emergence of new antigenic variants and reinforce the selection of vaccine viruses.
Issue number 6