Optimised oligonucleotide substrates to assay XPF-ERCC1 nuclease activity for the discovery of DNA repair inhibitors

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Abstract

We report the design and optimisation of novel oligonucleotide substrates for a sensitive fluorescence assay for high-throughput screening and functional studies of the DNA repair enzyme, XPF-ERCC1, with a view to accelerating inhibitor and drug discovery.

Journal details

Volume 55
Issue number 78
Pages 11671-11674
Available online
Publication date

Crick authors

Crick First author
Crick Corresponding author