Optimised oligonucleotide substrates to assay XPF-ERCC1 nuclease activity for the discovery of DNA repair inhibitors

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Adam M Thomas Sanja Brolih Joanna F McGouran Afaf H El-Sagheer Denis Ptchelkine Morgan Jones Neil McDonald Peter J McHugh Tom Brown
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Abstract

We report the design and optimisation of novel oligonucleotide substrates for a sensitive fluorescence assay for high-throughput screening and functional studies of the DNA repair enzyme, XPF-ERCC1, with a view to accelerating inhibitor and drug discovery.

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Journal Chemical Communications
Volume 55
Issue number 78
Pages 11671-11674
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Publisher website (DOI) 10.1039/c9cc05476f
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Europe PubMed Central 31497827
Pubmed 31497827

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