Optimised oligonucleotide substrates to assay XPF-ERCC1 nuclease activity for the discovery of DNA repair inhibitors.

Journal Article: Chemical CommunicationsYear Published: (2019) Volume Number: 55, Article Number: 11671-11674


Thomas,Adam M; Brolih,Sanja; McGouran,Joanna F; El-Sagheer,Afaf H; Ptchelkine,Denis; Jones,Morgan; McDonald,Neil Q; McHugh,Peter J; Brown,Tom

We report the design and optimisation of novel oligonucleotide substrates for a sensitive fluorescence assay for high-throughput screening and functional studies of the DNA repair enzyme, XPF-ERCC1, with a view to accelerating inhibitor and drug discovery.