p120-catenin prevents multinucleation through control of MKLP1-dependent RhoA activity during cytokinesisMore about Open Access at the Crick
Authors listRobert AH van de Ven Jolien S de Groot Danielle Park Robert van Domselaar Danielle de Jong Karoly Szuhai Elsken van der Wall Oscar M Rueda H Raza Ali Carlos Caldas Paul J van Diest Martin W Hetzer Erik Sahai Patrick WB Derksen
Spatiotemporal activation of RhoA and actomyosin contraction underpins cellular adhesion and division. Loss of cell-cell adhesion and chromosomal instability are cardinal events that drive tumour progression. Here, we show that p120-catenin (p120) not only controls cell-cell adhesion, but also acts as a critical regulator of cytokinesis. We find that p120 regulates actomyosin contractility through concomitant binding to RhoA and the centralspindlin component MKLP1, independent of cadherin association. In anaphase, p120 is enriched at the cleavage furrow where it binds MKLP1 to spatially control RhoA GTPase cycling. Binding of p120 to MKLP1 during cytokinesis depends on the N-terminal coiled-coil domain of p120 isoform 1A. Importantly, clinical data show that loss of p120 expression is a common event in breast cancer that strongly correlates with multinucleation and adverse patient survival. In summary, our study identifies p120 loss as a driver event of chromosomal instability in cancer.