PAWS1 controls Wnt signalling through association with casein kinase 1αMore about Open Access at the Crick
Authors listPolyxeni Bozatzi Kevin S Dingwell Kevin ZL Wu Fay Cooper Timothy D Cummins Luke D Hutchinson Janis Vogt Nicola T Wood Thomas J Macartney Joby Varghese Robert Gourlay David G Campbell Jim Smith Gopal P Sapkota
The BMP and Wnt signalling pathways determine axis specification during embryonic development. Our previous work has shown that PAWS1 (also known as FAM83G) interacts with SMAD1 and modulates BMP signalling. Here, surprisingly, we show that overexpression of PAWS1 in embryos activates Wnt signalling and causes complete axis duplication. Consistent with these observations in , Wnt signalling is diminished in U2OS osteosarcoma cells lacking PAWS1, while BMP signalling is unaffected. We show that PAWS1 interacts and co-localises with the α isoform of casein kinase 1 (CK1), and that PAWS1 mutations incapable of binding CK1 fail both to activate Wnt signalling and to elicit axis duplication in embryos.
Journal EMBO Reports
Issue number 4