Polθ inhibitors elicit BRCA-gene synthetic lethality and target PARP inhibitor resistanceMore about Open Access at the Crick
Authors listDiana Zatreanu Helen MR Robinson Omar Alkhatib Marie Boursier Harry Finch Lerin Geo Diego Grande Vera Grinkevich Robert A Heald Sophie Langdon Jayesh Majithiya Claire McWhirter Niall MB Martin Shaun Moore Joana Neves Eeson Rajendra Marco Ranzani Theresia Schaedler Martin Stockley Kimberley Wiggins Rachel Brough Sandhya Sridhar Aditi Gulati Nan Shao Luned M Badder Daniela Novo Eleanor G Knight Rebecca Marlow Syed Haider Elsa Callen Graeme Hewitt Joost Schimmel Remko Prevo Christina Alli Amanda Ferdinand Cameron Bell Peter Blencowe Chris Bot Mathew Calder Mark Charles Jayne Curry Tennyson Ekwuru Katherine Ewings Wojciech Krajewski Ellen MacDonald Hollie McCarron Leon Pang Chris Pedder Laurent Rigoreau Martin Swarbrick Ed Wheatley Simon Willis Ai Ching Wong Andre Nussenzweig Marcel Tijsterman Andrew Tutt Simon Boulton Geoff S Higgins Stephen J Pettitt Graeme CM Smith Christopher J Lord
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To identify approaches to target DNA repair vulnerabilities in cancer, we discovered nanomolar potent, selective, low molecular weight (MW), allosteric inhibitors of the polymerase function of DNA polymerase Polθ, including ART558. ART558 inhibits the major Polθ-mediated DNA repair process, Theta-Mediated End Joining, without targeting Non-Homologous End Joining. In addition, ART558 elicits DNA damage and synthetic lethality in BRCA1- or BRCA2-mutant tumour cells and enhances the effects of a PARP inhibitor. Genetic perturbation screening revealed that defects in the 53BP1/Shieldin complex, which cause PARP inhibitor resistance, result in in vitro and in vivo sensitivity to small molecule Polθ polymerase inhibitors. Mechanistically, ART558 increases biomarkers of single-stranded DNA and synthetic lethality in 53BP1-defective cells whilst the inhibition of DNA nucleases that promote end-resection reversed these effects, implicating these in the synthetic lethal mechanism-of-action. Taken together, these observations describe a drug class that elicits BRCA-gene synthetic lethality and PARP inhibitor synergy, as well as targeting a biomarker-defined mechanism of PARPi-resistance.
Journal Nature Communications
Issue number 1