Polycomb repressive complex PRC1 spatially constrains the mouse embryonic stem cell genomeMore about Open Access at the Crick
Authors listStefan Schoenfelder Robert Sugar Andrew Dimond Biola-Maria Javierre Harry Armstrong Borbala Mifsud Emilia Dimitrova Louise Matheson Filipe Tavares-Cadete Mayra Furlan-Magaril Anne Segonds-Pichon Wiktor Jurkowski Steven W Wingett Kristina Tabbada Simon Andrews Bram Herman Emily LeProust Cameron S Osborne Haruhiko Koseki Peter Fraser Nicholas Luscombe Sarah Elderkin
The Polycomb repressive complexes PRC1 and PRC2 maintain embryonic stem cell (ESC) pluripotency by silencing lineage-specifying developmental regulator genes. Emerging evidence suggests that Polycomb complexes act through controlling spatial genome organization. We show that PRC1 functions as a master regulator of mouse ESC genome architecture by organizing genes in three-dimensional interaction networks. The strongest spatial network is composed of the four Hox gene clusters and early developmental transcription factor genes, the majority of which contact poised enhancers. Removal of Polycomb repression leads to disruption of promoter-promoter contacts in the Hox gene network. In contrast, promoter-enhancer contacts are maintained in the absence of Polycomb repression, with accompanying widespread acquisition of active chromatin signatures at network enhancers and pronounced transcriptional upregulation of network genes. Thus, PRC1 physically constrains developmental transcription factor genes and their enhancers in a silenced but poised spatial network. We propose that the selective release of genes from this spatial network underlies cell fate specification during early embryonic development.
Journal Nature Genetics
Issue number 10