Preparing frozen-hydrated protein-nucleic acid assemblies for high-resolution cryo-EM imaging
Authors listPanchali Goswami Julia Locke Alessandro Costa
High-resolution image acquisition and structure determination by cryo-electron microscopy is becoming increasingly streamlined. Preparing electron-microscopy grids of suitable quality remains, however, a critical bottleneck. Strategies to achieve particle monodispersity, optimal sample concentration and suitable ice thickness can vary from specimen to specimen. In this book chapter we describe our protocols for negative-stain grid and cryo-grid preparation, which we apply to studying protein-nucleic acid complexes.
Journal Methods in Molecular Biology
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Publisher website (DOI) 10.1007/978-1-4939-8591-3_17
Europe PubMed Central 29956239
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