Quantifying autophagic structures in mammalian cells using confocal microscopy
Abstract
Autophagy relies on the sequential, hierarchical association of proteins with phagophores, and forming autophagosomes to allow completion of the process. Additionally, the trafficking of the unique transmembrane autophagy-related protein ATG9 is vital for autophagy progression. In this chapter, we discuss methods to monitor autophagosome number using confocal microscopy, by following the association of different autophagosomal markers with the phagophore and completed autophagosome. We also discuss methods to monitor the trafficking of ATG9 in mammalian cells under starvation conditions.
Journal details
Journal Methods in Enzymology
Volume 587
Pages 21-42
Available online
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Publisher website (DOI) 10.1016/bs.mie.2016.09.051
Europe PubMed Central 28253957
Pubmed 28253957
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