RECQL5 controls transcript elongation and suppresses genome instability associated with transcription stress
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Marco Saponaro Theodoros Kantidakis Richard Mitter Gavin Kelly Mark Heron Hannah Williams Johannes Söding Aengus Stewart Jesper SvejstrupAbstract
RECQL5 is the sole member of the RECQ family of helicases associated with RNA polymerase II (RNAPII). We now show that RECQL5 is a general elongation factor that is important for preserving genome stability during transcription. Depletion or overexpression of RECQL5 results in corresponding shifts in the genome-wide RNAPII density profile. Elongation is particularly affected, with RECQL5 depletion causing a striking increase in the average rate, concurrent with increased stalling, pausing, arrest, and/or backtracking (transcription stress). RECQL5 therefore controls the movement of RNAPII across genes. Loss of RECQL5 also results in the loss or gain of genomic regions, with the breakpoints of lost regions located in genes and common fragile sites. The chromosomal breakpoints overlap with areas of elevated transcription stress, suggesting that RECQL5 suppresses such stress and its detrimental effects, and thereby prevents genome instability in the transcribed region of genes.
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Publisher website (DOI) 10.1016/j.cell.2014.03.048
Europe PubMed Central 24836610
Pubmed 24836610
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