Reg4+ deep crypt secretory cells function as epithelial niche for Lgr5+ stem cells in colon
Authors list
Nobuo Sasaki Norman Sachs Kay Wiebrands Saskia IJ Ellenbroek Arianna Fumagalli Anna Lyubimova Harry Begthel Maaike van den Born Johan H van Es Wouter R Karthaus Vivian Li Carmen López-Iglesias Peter J Peters Jacco van Rheenen Alexander van Oudenaarden Hans CleversAbstract
Leucine-rich repeat-containing G-protein coupled receptor 5-positive (Lgr5(+)) stem cells reside at crypt bottoms of the small and large intestine. Small intestinal Paneth cells supply Wnt3, EGF, and Notch signals to neighboring Lgr5(+) stem cells. Whereas the colon lacks Paneth cells, deep crypt secretory (DCS) cells are intermingled with Lgr5(+) stem cells at crypt bottoms. Here, we report regenerating islet-derived family member 4 (Reg4) as a marker of DCS cells. To investigate a niche function, we eliminated DCS cells by using the diphtheria-toxin receptor gene knocked into the murine Reg4 locus. Ablation of DCS cells results in loss of stem cells from colonic crypts and disrupts gut homeostasis and colon organoid growth. In agreement, sorted Reg4(+) DCS cells promote organoid formation of single Lgr5(+) colon stem cells. DCS cells can be massively produced from Lgr5(+) colon stem cells in vitro by combined Notch inhibition and Wnt activation. We conclude that Reg4(+) DCS cells serve as Paneth cell equivalents in the colon crypt niche.
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Volume 113
Issue number 37
Pages E5399-E5407
Available online
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Publisher website (DOI) 10.1073/pnas.1607327113
Europe PubMed Central 27573849
Pubmed 27573849
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