Removing physiological motion from intravital and clinical functional imaging data
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Sean C Warren Max Nobis Astrid Magenau Yousuf H Mohammed David Herrmann Imogen Moran Claire Vennin James RW Conway Pauline Mélénec Thomas R Cox Yingxiao Wang Jennifer P Morton Heidi CE Welch Douglas Strathdee Kurt Anderson Tri Giang Phan Michael S Roberts Paul TimpsonAbstract
Intravital microscopy can provide unique insights into the function of biological processes in a native context. However, physiological motion caused by peristalsis, respiration and the heartbeat can present a significant challenge, particularly for functional readouts such as fluorescence lifetime imaging (FLIM), which require longer acquisition times to obtain a quantitative readout. Here, we present and benchmark , a versatile multi-platform software tool for image-based correction of sample motion blurring in both time resolved and conventional laser scanning fluorescence microscopy data in two and three dimensions. We show that is able to resolve intravital FLIM-FRET images of intra-abdominal organs in murine models and NADH autofluorescence of human dermal tissue imaging subject to a wide range of physiological motions. Thus, can enable FLIM imaging in situations where a stable imaging platform is not always possible and rescue previously discarded quantitative imaging data.
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Publisher website (DOI) 10.7554/eLife.35800
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Europe PubMed Central 29985127
Pubmed 29985127