Solution structures of the two PBZ domains from human APLF and their interaction with poly(ADP-ribose)
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Sebastian Eustermann Christoph Brockmann Pawan Vinod Mehrotra Ji-Chun Yang David Loakes Stephen West Ivan Ahel David NeuhausAbstract
Addition of poly(ADP-ribose) (PAR) is an important post-translational modification in higher eukaryotes. Several DNA repair and checkpoint proteins possess specific PAR-binding zinc-finger (PBZ) modules critical for function. Here, we present solution structures of the two PBZ modules of aprataxin and PNK-like factor (APLF), revealing a novel type of zinc finger. By combining in vivo PAR-binding data with NMR interaction data using PAR fragments, we propose a structural basis for PBZ-PAR recognition.
Journal details
Volume 17
Issue number 2
Pages 241-243
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Publisher website (DOI) 10.1038/nsmb.1747
Europe PubMed Central 20098424
Pubmed 20098424
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