Spatiotemporal regulation of Aurora B recruitment ensures release of cohesion during C. elegans oocyte meiosis
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Nuria Ferrandiz Consuelo Barroso Oana Telecan Nan Shao Hyun-Min Kim Sarah Testori Peter Faull Pedro Cutillas Bram Snijders Monica P Colaiácovo Enrique Martinez-PerezAbstract
The formation of haploid gametes from diploid germ cells requires the regulated two-step release of sister chromatid cohesion (SCC) during the meiotic divisions. Here, we show that phosphorylation of cohesin subunit REC-8 by Aurora B promotes SCC release at anaphase I onset in C. elegans oocytes. Aurora B loading to chromatin displaying Haspin-mediated H3 T3 phosphorylation induces spatially restricted REC-8 phosphorylation, preventing full SCC release during anaphase I. H3 T3 phosphorylation is locally antagonized by protein phosphatase 1, which is recruited to chromosomes by HTP-1/2 and LAB-1. Mutating the N terminus of HTP-1 causes ectopic H3 T3 phosphorylation, triggering precocious SCC release without impairing earlier HTP-1 roles in homolog pairing and recombination. CDK-1 exerts temporal regulation of Aurora B recruitment, coupling REC-8 phosphorylation to oocyte maturation. Our findings elucidate a complex regulatory network that uses chromosome axis components, H3 T3 phosphorylation, and cell cycle regulators to ensure accurate chromosome segregation during oogenesis.
Journal details
Journal Nature Communications
Volume 9
Issue number 1
Pages 834
Available online
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Publisher website (DOI) 10.1038/s41467-018-03229-5
Europe PubMed Central 29483514
Pubmed 29483514
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