Stress granules plug and stabilize damaged endolysosomal membranes
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Claudio Bussi Agustín Mangiarotti Christian Vanhille-Campos Beren Aylan Enrica Pellegrino Natalia Athanasiadi Tony Fearns Angela Rodgers Titus M Franzmann Anđela Šarić Rumiana Dimova Maximiliano GutierrezAbstract
Endomembrane damage represents a form of stress that is detrimental for eukaryotic cells1,2. To cope with this threat, cells possess mechanisms that repair the damage and restore cellular homeostasis3-7. Endomembrane damage also results in organelle instability and the mechanisms by which cells stabilize damaged endomembranes to enable membrane repair remains unknown. Here, by combining in vitro and in cellulo studies with computational modelling we uncover a biological function for stress granules whereby these biomolecular condensates form rapidly at endomembrane damage sites and act as a plug that stabilizes the ruptured membrane. Functionally, we demonstrate that stress granule formation and membrane stabilization enable efficient repair of damaged endolysosomes, through both ESCRT (endosomal sorting complex required for transport)-dependent and independent mechanisms. We also show that blocking stress granule formation in human macrophages creates a permissive environment for Mycobacterium tuberculosis, a human pathogen that exploits endomembrane damage to survive within the host.
Journal details
Journal
Nature
Volume
623
Issue number
7989
Pages
1062-1069
Available online
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10.1038/s41586-023-06726-w
Europe PubMed Central
37968398
Pubmed
37968398
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