Structural insights into Escherichia coli phosphopantothenoylcysteine synthetase by native ion mobility-mass spectrometry

Authors list

Daniel Shiu-Hin Chan Jeannine Hess Elen Shaw Christina Spry Robert Starley Claudio Dagostin Marcio VB Dias Ramesh Kale Vitor Mendes Tom L Blundell Anthony G Coyne Chris Abell
Scroll for detail on labs, facilities & authors

Abstract

CoaBC, part of the vital coenzyme A biosynthetic pathway in bacteria, has recently been validated as a promising antimicrobial target. In this work, we employed native ion mobility-mass spectrometry to gain structural insights into the phosphopantothenoylcysteine synthetase domain of E. coli CoaBC. Moreover, native mass spectrometry was validated as a screening tool to identify novel inhibitors of this enzyme, highlighting the utility and versatility of this technique both for structural biology and for drug discovery.

Journal details

Journal Biochemical Journal
Volume 476
Issue number 21
Pages 3125-3139
Available online
Publication date

Full text links

Publisher website (DOI) 10.1042/BCJ20190318
Europe PubMed Central 31488574
Pubmed 31488574

Keywords

Type of publication