Synthetic physical interactions with the yeast centrosome
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Rowan SM Howell Attila Csikász-Nagy Peter H ThorpeAbstract
The yeast centrosome or Spindle Pole Body (SPB) is an organelle situated in the nuclear membrane, where it nucleates spindle microtubules and acts as a signaling hub. Various studies have explored the effects of forcing individual proteins to interact with the yeast SPB, however no systematic study has been performed. We used synthetic physical interactions to detect proteins that inhibit growth when forced to associate with the SPB. We found the SPB to be especially sensitive to relocalization, necessitating a novel data analysis approach. This novel analysis of SPI screening data shows that regions of the cell are locally more sensitive to forced relocalization than previously thought. Furthermore, we found a set of associations that result in elevated SPB number and, in some cases, multi-polar spindles. Since hyper-proliferation of centrosomes is a hallmark of cancer cells, these associations point the way for the use of yeast models in the study of spindle formation and chromosome segregation in cancer.
Journal details
Journal G3 : Genes, Genomes, Genetics
Volume 9
Issue number 7
Pages 2183-2194
Available online
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Publisher website (DOI) 10.1534/g3.119.400117
Europe PubMed Central 31076383
Pubmed 31076383
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