Tailoring an alien ferredoxin to support native-like P450 monooxygenase activity

Abstract

A ferredoxin associated with biological Fe-S cluster assembly has been remodelled to transfer electrons to a P450 enzyme and support substrate oxidation at 80% of the physiological ferredoxin activity, opening up the possibility of tailoring ferredoxins to reconstitute the activity of P450 enzymes for which the electron transfer partner proteins are not known.

Journal details

Volume 48
Issue number 95
Pages 11692-11694
Available online
Publication date

Keywords