Targeted in vivo electroporation using nanoengineered microelectrodes.

Journal Article: Methods in Molecular BiologyYear Published: (2020) Volume Number: 2050, Article Number: 113-120

Authors

Schwarz,Daniel; Schaefer,Andreas T

Targeted electroporation by using glass microelectrodes is a popular and versatile tool allowing for easy manipulation of single cells and cell ensembles in living tissue. Because of the highly focal distribution of the electric field, however, the range of reversible electroporation without causing irreversible damage is tight-especially when aiming for larger electroporation volumes. In this chapter, we describe the production of nanoengineered electroporation microelectrodes (NEMs), a practicable way to prepare glass microelectrodes providing a more even distribution around the tip of a pipette by using nanotechnological methods.