Targeting Alp7/TACC to the spindle pole body is essential for mitotic spindle assembly in fission yeast
Authors list
Ngang Heok Tang Naoyuki Okada Chii Shyang Fong Kunio Arai Masamitsu Sato Takashi TodaAbstract
The conserved TACC protein family localises to the centrosome (the spindle pole body, SPB in fungi) and mitotic spindles, thereby playing a crucial role in bipolar spindle assembly. However, it remains elusive how TACC proteins are recruited to the centrosome/SPB. Here, using fission yeast Alp7/TACC, we have determined clustered five amino acid residues within the TACC domain required for SPB localisation. Critically, these sequences are essential for the functions of Alp7, including proper spindle formation and mitotic progression. Moreover, we have identified pericentrin-like Pcp1 as a loading factor to the mitotic SPB, although Pcp1 is not a sole platform.
Full text links
Publisher website (DOI) 10.1016/j.febslet.2014.06.027
Europe PubMed Central 24937146
Pubmed 24937146
Keywords
Type of publication