Tbx4 function during hindlimb development reveals a mechanism that explains the origins of proximal limb defects
More about Open Access at the CrickAuthors list
Veronique Duboc Fatima A Sulaiman Eleanor Feneck Ania Kucharska Donald Bell Muriel Holder-Espinasse Malcolm PO LoganAbstract
We dissect genetically a gene regulatory network, including the transcription factors Tbx4, Pitx1 and Isl1 that act cooperatively to establish the hindlimb bud and identify key differences in the pathways that initiate formation of the hindlimb and forelimb. Using live image analysis of limb mesenchyme cells undergoing chondrogenesis in micromass culture, we distinguish a series of changes in cellular behaviours and cohesiveness that are required for chondrogenic precursors to undergo differentiation. Furthermore, we provide evidence that the proximal hindlimb defects in the Tbx4 mutant result from a failure in the early differentiation step of chondroprogenitors into chondrocytes, providing a novel explanation for the origins of proximally-biased limb defects.
Journal details
Journal Development
Volume 148
Issue number 19
Pages dev199580
Available online
Publication date
Full text links
Publisher website (DOI) 10.1242/dev.199580
Europe PubMed Central 34423345
Pubmed 34423345