The ALS- and FTD-associated proteins annexin A11 and CHMP2B act sequentially in plasma membrane repair More about Open Access at the Crick
Authors list
Catherine Heffner Georgina Starling Lorian Straker Pippa Hawes Adrian M Isaacs Jeremy CarltonAbstract
Maintenance of plasma membrane integrity is essential for compartmentalization of the cytosol and for cellular viability. Upon membrane damage, several factors including endosomal sorting complex required for transport-III (ESCRT-III) proteins, annexins, stress granules, lipids, and membrane fusion proteins are mobilized to orchestrate membrane repair. However, whether these factors operate independently or act together is unclear. Here, using human cell lines, we expose temporal differences and interdependencies in the recruitment of ESCRT-III and annexin proteins to sites of plasma membrane damage. We show that annexin proteins are recruited immediately and form a plug at the damage site, restricting membrane permeability. We find that ESCRT-III assembles later and acts to release plug-containing damaged membranes from the cell. Further, frontotemporal dementia (FTD)- and amyotrophic lateral sclerosis (ALS)-associated mutations in the ESCRT-III protein, CHMP2B, and the annexin protein, ANXA11, compromise plasma membrane repair, suggesting that defects in this process may contribute to these pathologies. These data present an integrated "sealing and healing" model of membrane repair.
Journal details
Journal
Developmental Cell
Volume
61
Issue number
7
Pages
1487-1503.e7
Available online
Publication date
Full text links
Publisher website (DOI)
10.1016/j.devcel.2026.05.014
Figshare
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Europe PubMed Central
42349418
Pubmed
42349418
Publishing history
The publication was previously a preprint.
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