The centriolar satellite protein Cep131 is important for genome stability
Authors list
Christopher J Staples Katie N Myers Ryan DD Beveridge Abhijit A Patil Alvin Lee Charles Swanton Michael Howell Simon Boulton Spencer J CollisAbstract
The centrosome acts as a centre for microtubule organisation and plays crucial roles in cell polarity, migration, growth and division. Cep131 has recently been described as a basal body component essential for cilium formation, but its function in non-ciliogenic cells is unknown. We identified human Cep131 (also known as AZI1) in a screen for regulators of genome stability. We show that centrosomal localisation of Cep131 is cell-cycle-regulated and requires both an intact microtubule network and a functional dynein-dynactin transport system. Cep131 is recruited to centriolar satellites by PCM1, and localised to the centriolar core region by both pericentrin and Cep290. Depletion of Cep131 results in a reduction in proliferation rate, centriole amplification, an increased frequency of multipolar mitosis, chromosomal instability and an increase in post-mitotic DNA damage. These data therefore highlight the importance of human Cep131 for maintaining genomic integrity.
Journal details
Journal Journal of Cell Science
Volume 125
Issue number 20
Pages 4770-4779
Publication date
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Publisher website (DOI) 10.1242/jcs.104059
Europe PubMed Central 22797915
Pubmed 22797915
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