The Streptococcus pneumoniae transcriptome in patient cerebrospinal fluid identifies novel virulence factors required for meningitis
Authors list
Emma Wall José Afonso Guerra-Assunção Marie Yang Rutger Konig Teerawit Audshasai Alize Proust Rieza Aprianto Elisa Ramos-Sevillano Giuseppe Ercoli Nicola Bordin Vanessa S Terra Jan-Willem Veening David G Lalloo Brendan W Wren Robert Wilkinson Matthijs C Brouwer Diederik van de Beek Aras Kadiloglu Robert S Heyderman Jeremy S BrownThis article is a preprint. Preprints have not been peer-reviewed.
You can read more about preprints.
Abstract
To better understand Streptococcus pneumoniae pathogenesis we performed RNA sequencing on cerebrospinal fluid (CSF) from meningitis patients to identify bacterial genes expressed during invasion of the central nervous system. Comparison to transcriptome data for serotype 1 S. pneumoniae cultured in ex vivo human CSF defined a subset of 57 genes with high expression during human meningitis. Deletion of two of the most highly expressed genetic loci, bgaA (encodes for a beta-galactosidase) or the SP_1801-5 putative stress response operon, resulted in S. pneumoniae strains still able to transmigrate the blood brain barrier but which were more susceptible to complement opsonisation and unable to maintain brain infection in a murine meningitis model. In 1144 meningitis patients, infection with bgaA containing S. pneumoniae strains was associated with a higher mortality (22% versus 14% p=0.02). These data demonstrate that direct bacterial RNAseq from CSF can identify previously undescribed S. pneumoniae virulence factors required for meningitis pathogenesis.
Details
Archive
bioRxiv
Available online
Publication date
Keywords
Type of publication
