UV irradiation induces a non-coding RNA that functionally opposes the protein encoded by the same geneMore about Open Access at the Crick
Authors listLaura Williamson Marco Saponaro Stefan Boeing Phil East Richard Mitter Theodoros Kantidakis Gavin Kelly Anna Lobley Jane Walker Bradley Spencer-Dene Michael Howell Aengus Stewart Jesper Svejstrup
The transcription-related DNA damage response was analyzed on a genome-wide scale with great spatial and temporal resolution. Upon UV irradiation, a slowdown of transcript elongation and restriction of gene activity to the promoter-proximal ∼25 kb is observed. This is associated with a shift from expression of long mRNAs to shorter isoforms, incorporating alternative last exons (ALEs) that are more proximal to the transcription start site. Notably, this includes a shift from a protein-coding ASCC3 mRNA to a shorter ALE isoform of which the RNA, rather than an encoded protein, is critical for the eventual recovery of transcription. The non-coding ASCC3 isoform counteracts the function of the protein-coding isoform, indicating crosstalk between them. Thus, the ASCC3 gene expresses both coding and non-coding transcript isoforms with opposite effects on transcription recovery after UV-induced DNA damage.
Issue number 5