This three-day course will serve as an introduction to the use of flow cytometry to delegates who have some flow cytometry experience and who wish to expand their knowledge.
We will cover the basics of fluorescence and the flow cytometer and demonstrate some of the more commonly used applications to illustrate how the cytometer can be used. We will cover multicolour fluorescence and compensation, DNA and cell cycle kinetics, cell death and apoptosis and receptor quantification. Delegates will have the opportunities to gain hands-on experience using either a BDLSRII or an LSR Fortessa and data generated will also be used to help illustrate how best to perform data analysis.
Places will be limited to 12 delegates to ensure maximum interaction. The lead tutor will be Derek Davies.
For further information, contact firstname.lastname@example.org.
- Instrument set-up
- Compensation using beads
- Compensation experiments
- Viability dyes and DNA analysis
- Data analysis
- Cell death
- Antibody titration
- Dye dilution for proliferation
- Fluorescence and fluorochromes
- How a cytometer works
- DNA and cell cycle analysis
- Cell proliferation